New Staining techniques can now visualize the whole organs and Body
RIKEN research team has now established a very innovative and optimized 3_D tissue staining and observation technique which is based on existing tissue clearing technology. This new technique can be used to label the tissues in mouse, human and whole marmoset bodies, it will allow the detailed anatomical study and the comparison of whole organs between species at cellular level.
RIKEN, Center for Biosystems and dynamics Research has developed a 3_D technique called CUBIC. Which can make a 3_D image of every cell by making the tissue transparent.
By clearing tissue technique we get fantastical images, it doesn't have any scientific value itself. This requires a system which uses different antibodies and staining agents for labeling the specific tissues or cell types. The researchers found that the biological tissues can be defined as a type of electrolyte gel.
Based on the properties of tissues, they have constructed a screening system in order to examine the different conditions using artificial gels that can mimic the tissues.
When the artificial gels were analyzed and stained by CUBIC, they were able to establish a fine tuned 3_D imaging method.
The system uses about 30 different antibodies and different nuclear staining agents this helps the scientists almost in every field; one can study the kidney cells to brain cells function through this technique.
This technique is far known as CUBIC histovision, which has also revealed the distribution patterns of blood vessels, brain cells of mouse and also the distribution of glia cells in cerebellum of brain of human and mice .The 3_D staining method is now considered to be the world best method, as it also has found the paradigm shift in the development methods of tissue chemistry. These results are not to able to reveal the understanding methods of biological systems at organ and organism level, it will also help in improvement of diagnostic accuracy and also in 3_D clinical pathology examination.